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Center for Mass Spectrometry

STAFF

Athula Attygalle Athula Attygalle
Position: Professor of Chemistry
Office: McLean  Building, Room 312
E-mail: aattygal@stevens.edu
Phone: 201-216-5575,  201-216-8378
Fax: 201-216-8240,  201-216-8196
Research Interests: Mass Spectrometry, Organic Chemistry, Microchemical techniques, Chromatography, GC-Infrared Spectrometry. Natural Products, Chemical Communication and Defense in Arthropods, Chemical Ecology, “Chemical Prospecting” by Pharmacological Screening of Tropical Rain Forest Natural Products.
Publications  
Josef Ruzicka
Position: Postdoctoral Research Fellow
Office: McLean  Building, Room 508
E-mail: jruzick1@stevens.edu
Phone: 201-216-8101
Fax: 201-216-8240
Research Interests: Mass Spectrometry, Fragmentation mechanisms, Micro-scale Synthetic Organic Chemistry, Stable isotope labeled compounds.
Education:

2001 Doctorate Degree in Organic ChemistryCharles University, Faculty of Science, Prague, Czech Republic Academy of Sciences of the Czech Republic, Institute of Organic Chemistry and Biochemistry

1997 Master of Science Degree in Organic ChemistryCharles University, Faculty of Science, Prague, Czech Republic

1995 Bachelor of Science Degree in ChemistryCharles University, Faculty of Science, Prague, Czech Republic

Publications:

1. Ruzicka J., Streinz L., Saman D., Havlas Z., Wimmer Z., Zarevucka,M., Koutek B., Leseticky L., Chlorofluoroacetic Acid as a Highly Versatile Derivatizing Agent: Assignment of Stereochemistry to Esters of Chiral Alcohols, Collect. Czech. Chem. Commun. 2000, 65, 695

2. Ruzicka J., Koutek B., Streinz L., Saman D., Leseticky L., A new access of b-methyl substituted secondary alcohols. Application to the synthesis of 4-methylheptan-3-ol, Tetrahedron: Asymmetry 1999, 10, 3521

3. Ruzicka J., Streinz L., Wimmer Z., Rejzek M., Zarevucka M., Koutek M., Leseticky L., Chlorofluoroacetic Acid Derivatives of Sterically Hindered Chiral Alcohols, J. Chem. Res., Synopses 1998, 830, Miniprint 1998, 3401

Wu, Xiaogang
Position: Research Assistant (Graduate Student)
Office: McLean  Building, Room 508
E-mail: xwu1@stevens.edu
Phone: 201-216-8101
Fax: 201-216-8240
Research Interests: Mass Spectrometry, Microchemical techniques, Chromatography, GC-Infrared Spectrometry. Natural Products, Chemical Communication and Defense in Arthropods, Chemical Ecology.
Education:
Master of Science, Central China Normal University(CCNU) Bachelor of Science, CCNU
Publications:

1. Wu Xiaogang, Lu Guanghan, Anodic voltammetric behavior of iodide on a chitosan-modified glassy carbon electrode,Analytical Letters, 2001, 34,1205 - 1214

2. Lu Guanghan, Yao Xin, Wu Xiaogang,  Determination of the total   iron by chitosan-modified glassy carbon electrode, Microchemical Journal, 2001, 69,81-87

3. Yao Xin, Lu Guanghan, Wu Xiaogang,  Studies on Electrochemical Behavior of Bromine at a Chitosan-modified Glassy Carbon Electrode Electroanalysis, 2001,13, 923-926

4. Lu Guanghan, Wu Xiaogang, Studies on 1:12 phosphomolybdic heteropoly anion film modified carbon paste electrode. Talanta, 1999, 49, 511-515

5. Lu Guanghan*, Wang Qionglin, Wu Xiaogang, etal. Polarographic determination of trace fluoride in foods. Food Chemistry, 1999, 66, 519-523 

6. Li Qiong, Lu Guanghan, Wu Heng, Xiaogang Wu. Determination of trace tin in foods by single-sweep polarography. Food Chemistry, 1999, 64, 129-132

7. Wu Xiaogang, The electroanalytical studies of 1:12 phosphomolybdic hetropoly acid film modified electrode. J. Central China Normal Univ.(Nat. Sci), 1998, 22, 323-326

8. Wu Xiaogang,  Application of polarographic analysis in environmental samples. Environmental Science and Technology, 1998, No.2, 27-32.

Vaclav Cerovsky
Position: Postdoctoral Research Fellow
Office: McLean  Building, Room 508
E-mail: vcerovsk@stevens.edu
Phone: 201-216-8101
Fax: 201-216-8240
Research Interests: Protein Chemistry, HPLC, Isolation of natural Products
Education:

1974-79  University study: Charles University, Prague

1978-79  Department of Biochemistry,  Charles University, Prague

1980-83  Ph.D. Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague  (Supervisor: Dr. Karel Jost) 

Thesis: "Application of enzymes in peptide synthesis"

1983-1993  Research associate at the Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague

1986 (1 month), 1987 (1 month) Visiting scholar:   Institute of Protein Research, Academy of Sciences of the USSR, Moscow Region, USSR, and Laboratory of Peptide Synthesis, Institute of Cardiology, Moscow, USSR

1989-90 (15 months) Postdoctoral study: Department of Biochemistry, Indiana University Purdue University at Indianapolis, Indianapolis, USA

(Prof. Dr. Anna DePaoli Roach)

1992-93 (18 months) Alexander von Humboldt fellow (Leipzig University, Leipzig, Germany. (Prof. Dr. Hans-Dieter Jakubke)

1993-2000  Senior scientist and the leader of a small research group at the Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic.

1993-1995 Joint work with Lonza AG, Switzerland

1997 (6 months) Follow-up-program of the Alexander von Humbold Foundation. Institute für Enzymtechnologie, Forschungszentrum Jülich.

1998 (5 months), 1999 (4 months) Scholarships from German Scientific Society (Deutsche Forschungsgemeinschaft), Leipzig University

Project leader: Dr. Frank Bordusa

2000 (3 months) Scholarship from Max-Planck-Society, Max-Planck Research Unit, Enzymology of Protein Folding, Halle/Saale, Germany

2001-2003 (two years) Visiting scientist at the Department of Chemistry and Chemical biology, Cornell University, Ithaca, NY, USA (Prof. Harold A. Scheraga)

Publications:

Interaction of trypsin with immobilized p-aminobenzamidine derivatives studied by means of affinity electrophoresis. Cerovsky V., Ticha M., Turkova J., Labsky J. J. Chromatogr. 1980, 194, 175-181.

Studies on lectins. XLIX. The use of glycosyl derivatives of Dextran T-500 for affinity electrophoresis of lectins. Cerovsky V, Ticha M., Horejsi V., Kocourek J. Biochem. Biophys. Methods 1980, 3, 163-172. 

Papain-catalyzed synthesis of phenylhydrazides of N-acylamino acids. Cerovsky V., Jost K. Collect. Czech. Chem. Commun. 1984, 49, 2557-2561.

Enzymatic resolution of g-carboxy-DL-glutamic acid. Cerovsky V., Jost K. Collect. Czech. Chem. Commun. 1984, 49, 2562-2565.

Enzymatically catalyzed synthesis of dipeptides of g-carboxy-L-glutamic acid from benzyloxycarbonyl-g-carboxy-DL-glutamic acid.Cerovsky V., Jost K. Collect. Czech. Chem. Commun. 1985, 50, 878-884.

Applicability of glutaminyl-tRNA cyclotransferase in the peptide synthesis. Cerovsky V., Jost K. Collect. Czech. Chem. Commun. 1985, 50, 2310-2318.

Enzymatically catalyzed synthesis of oxytocin fragments 1-6 and 7-9. Cerovsky V., Jost K. Collect. Czech. Chem. Commun. 1985, 50, 2775-2782. Synthesis of pressinoic acid by enzymatically catalyzed formation of peptide bonds.Cerovsky V. Collect. Czech. Chem. Commun. 1986, 51, 1352-1360.

Enzymatically catalyzed synthesis of dipeptides containing O4-sulfotyrosine and O4-phosphotyrosine. Cerovsky V., Jost K.  Collect. Czech. Chem. Commun. 1987, 52, 1079-1086.

Papain-catalyzed synthesis of 2-naphtylamides of N-acylamino acids and dipeptides.Cerovsky V., Saks T., Jost K. Collect. Czech. Chem. Commun. 1987, 52, 2309-2316. 

Synthesis of the carboxy-terminal octapeptide of cholecystokinin (CCK-8) based on incorporation of O4-sulfotyrosine by enzymatically catalyzed formation of peptide bonds. Cerovsky V., Hlavacek J., Slaninova J., Jost K. Collect. Czech. Chem. Commun. 1988, 53, 1086-1093.

Enzymes in preparative organic synthesis: The problem of increasing the yield of product. Semenov A.N., Cerovsky V., Titov M.I., Martinek K. Collect. Czech. Chem. Commun. 1988, 53, 2963-2985.

Free chymotrypsin-catalyzed synthesis of peptide bond in aliphatic alcohols with low water content. Cerovsky V., Martinek K. Collect. Czech. Chem. Commun. 1989, 54, 266-276.

Peptide synthesis catalyzed by native proteinase K in water-miscible organic solvents with low water content. Cerovsky V., Martinek K.  Collect. Czech. Chem. Commun. 1989, 54, 2027-2041.

Papain-catalyzed fragment synthesis of protected cholecystokinin derivatives. Cerovsky V., Pirkova J., Majer P., Slaninova J, Hlavacek J.  Collect. Czech. Chem. Commun. 1990, 55, 1873-1882.

Free trypsin-catalyzed peptide synthesis in acetonitrile with low water content. Cerovsky V.  Biotechnol. Lett. 1990, 12, 899-904.

V8 proteinase-catalyzed peptide synthesis in hydrophilic organic solvents with low water content. Cerovsky V. Tetrahedron Lett. 1991, 32, 3421-3424.

Serine proteinase-catalyzed incorporation of D-amino acids into model peptides in acetonitrile with low water content. Cerovsky V. Biomed. Biochim. Acta 1991, 50, 44-49.

Protease-catalysed peptide synthesis in solvent-free system. Cerovsky V. Biotechnol. Tech. 1992, 6, 155-160.

S`-Subsite mapping of polyethylene glycol-modified a-chymotrypsin and a-chymotrypsin: a comparative study. Cerovsky V., Ullmann D., Jakubke H.-D. Biochim. Biophys. Acta 1994, 1204, 91-96.

Acyl transfer reactions catalyzed by native and modified a-chymotrypsin in acetonitrile with low water content. Cerovsky V., Jakubke H.-D. Enzyme. Microb. Technol. 1994, 16, 596-601.

Enzymatic approach to the synthesis of taurine-containing peptides. Cerovsky V., Jakubke H.-D. Int. J. Peptide Protein Res. 1994, 44, 466-471.

Peptide synthesis catalyzed by crosslinked a-chymotrypsin in water/dimethylformamide system. Cerovsky V., Jakubke H.-D. Biocatalysis 1994, 11, 233-240.

Serine/threonine protein phosphatases in the control of cell function. DePaoli-Roach A., Park I.-K., Cerovsky V., Csortos S., Durbin S.D., Kuntz M.J., Sitikov A., Tang P.M., Verin A., Zolnierowicz S. Adv. Enzyme Regul. 1994, 34, 199-224.

Nucleophile specificity of subtilisin in an organic solvent with low water content. Investigation via acyl transfer reactions. Cerovsky V., Jakubke H.-D. Biotechnol. Bioeng. 1996, 49, 553-558.

Protease-catalyzed synthesis of Leu-enkephalin in solvent free system. Klein J. U., Cerovsky V. Int. J. Peptide Protein Res.  1996, 47, 348-352.

Enzymatic semisynthesis of dicarba-analogs of calcitonin. Cerovsky V., Wunsch E., Brass J. Eur. J. Biochem. 1997, 247, 231-237.

Resolution of DL-2-aminosuberic acid via protease-catalyzed ester hydrolysis. Rivard M., Malon P., Cerovsky V. Amino Acids 15, 1998, 389-392.

C-Terminal peptide amidation catalyzed by orange flavedo peptide amidase. Cerovsky V., Kula M.-R. Angew. Chem. Int. Ed. 1998, 37, 1885-1887. Angew. Chem. 1998, 110, 1186-1989.

Peptides of 2-Aminosuberic Acid I. New intermediates for synthetic studies. Wunsch E., Stocker H., Malon P., Gut V., Cerovsky V., Zertova M., Laffan D.D.  Amino Acids 2000, 18, 219-227.

Protease-catalyzed fragment condensation via substrate mimetic strategy: a useful combination of solid-phase peptide synthesis with enzymatic methods. Cerovsky V., Bordusa F. J. Peptide Res. 2000, 55, 325-329.

The applicability of subtilisin Carlsberg in peptide synthesis. Klein J.-U., Prykhodzka A., Cerovsky V. J. Peptide Sci. 2000, 6, 541-549.

Semisynthesis of Ht 31(493-515): Involvement  of PKA-anchoring proteins in the regulation of the cAMP-dependent chloride current in heart cells. Cerovsky V.,  Kockskaemper J., Glitsch H.G., Bordusa F. ChemBioChem  2000, 1, 126-129.

Studies on the peptide amidase-catalyzed peptide amidation in organic media with respect to its substrate specificity. Cerovsky V., Kula M.-R.  Biotechnol. Appl. Biochem. 2001, 33, 183-187

A convenient incorporation of conformationally constrained 5,5-dimethylproline into the ribonuclease A 89-124 sequence by condensation of synthetic peptide fragments. Cerovsky V., Welker E., Scheraga H.A. J. Peptide Res. 2003, 61, 140-151

Eva Kaufman
Position: Postdoctoral Research FellowFeodor Lynen Research Fellow of Alexander von Humboldt  Foundation
Office: McLean  Building, Room 508
E-mail: ekaufman@stevens.edu   
Phone: 201-216-8101
Fax: 201-216-8240
Research Interests: Entomology
Education:

J. W. Goethe-University, Frankfurt, Germany.  2003

Doctoral Thesis: “Southeast Asian Ant-Gardens: Diversity, ecology, ecological significance and evolution of mutualistic ant-epiphyte associations” 

J. W. Goethe-University, Frankfurt, GermanyOct. 1994 ­ May

 1998 “Diplom-Biologin” (comparable to Master of Science) in Biological

 Science.  Thesis: “Ant gardens on giant bamboo (Gigantochloa

 scortechinii, Poaceae) in West-Malaysia”

J. W. Goethe-University, Frankfurt, Germany. Oct. 1992 ­ July 1994

“Vordiplom” (comparable to bachelor’s degree) in Zoology, Botany,

Chemistry and Physics.

Publications:

1. Kaufmann, E., A. Weissflog, R. Hashim, and U. Maschwitz, 2001. Ant gardens on the giant bamboo Gigantochloa scortechinii (Poaceae) in West-Malaysia. Insectes soc. 48: 125-133.

2. Malsch, A.K.F., E. Kaufmann, H.P. Heckroth, D.J. Williams, M. Maryati, and U. Maschwitz, 2001. First report on root mealy bug (Homoptera: Pseudococcidae) manipulation and sociobiology of Pseudolasius spp. (Hymenoptera: Formicidae). Insectes soc. 48: 333-341.

3. Buschinger, A., U. Maschwitz and E. Kaufmann, 2002. Kein Kühlschrank? No problem! Wie Crematogaster-Ameisen in den Feuchttropen das Problem der Vorratshaltung lösen. Ameisenschutz aktuell 16: 1-5.

4. Kaufmann, E., A.K.F. Malsch, M. Erle and U. Maschwitz, in press. Compound nesting of Strumigenys sp. (Myrmicinae) and Diacamma sp. (Ponerinae), and other nesting symbioses of myrmicine and ponerine ants in Southeast Asia. Insectes soc.

5. Kaufmann, E., A.K.F. Malsch, D.J. Williams and U. Maschwitz, in press. Pseudolasius spp. and their trophobionts ­ Codispersal by colony-budding. Sociobiology

6. Kaufmann, E. and U. Maschwitz, 2002. The ‘ant epiphyte puzzle’ of tropical Asian rain forests. In: XIV International Congress of IUSSI, The Golden Jubilee Proceedings. Hokkaido University Press, Sapporo, Japan. p. 3

7. Hashim, R., S.H. Yek, E. Kaufmann, T. Akino, J. Billen and F. Ito (2002): Mimicry in an arboreal ant in Southeast Asia: Camponotus sp., a possible batesian mimic of Crematogaster inflata. In: XIV International Congress of IUSSI, The Golden Jubilee Proceedings. Hokkaido University Press, Sapporo, Japan. p. 196

Kaufmann, E. and U. Maschwitz, submitted. Gardener ants and their tree gardens: How to understand the ant-epiphyte puzzle of Asian tropical rain forests. Behav. Ecol. Sociobiol.

Maschwitz, U., C. Go, E. Kaufmann and A. Buschinger, submitted. A unique strategy of host colony exploitation in a parasitic ant: Workers of Polyrhachis lama rear the brood of their colony in neighboring host nests. Naturwissenschaften

 
   

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